Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
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Validation data
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- Product number
- PA5-52054 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PYGL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VVAATLQDII RRFKASKFGS TRGAGTVFDA FPDQVAIQLN DTHPALAIPE LMRIFVDIEK LPWSKAWELT QKTFAYTNHT VLPEALERWP VDLVEKLLPR HLEIIYEINQ KHLDRIVALF PKDVDRLRRM SLI Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 93%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PYGL in human cell lines PC-3 and SK-MEL-30 using a PYGL Polyclonal Antibody (Product # PA5-52054). Corresponding PYGL RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PYGL in human cell line U-2 OS shows positivity in plasma membrane & cytoplasm. Samples were probed using a PYGL Polyclonal Antibody (Product # PA5-52054).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human cerebral cortex using PYGL Polyclonal Antibody (Product # PA5-52054).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human liver tissue shows strong cytoplasmic positivity in hepatocytes. Samples were probed using a PYGL Polyclonal Antibody (Product # PA5-52054).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human liver and pancreas tissues using PYGL Polyclonal Antibody (Product # PA5-52054). Corresponding PYGL RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human bone marrow, cerebral cortex, liver and testis using PYGL Polyclonal Antibody (Product # PA5-52054) (A) shows similar protein distribution across tissues to an independent PYGL Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human pancreas using PYGL Polyclonal Antibody (Product # PA5-52054) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human bone marrow using PYGL Polyclonal Antibody (Product # PA5-52054).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PYGL in human testis using PYGL Polyclonal Antibody (Product # PA5-52054).