21698-1-AP

antibody from Proteintech Group

Targeting: NEDD4 KIAA0093, MGC176705, NEDD4-1, RPF1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21698-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#21698-1-AP, RRID:AB_10858626

Product name

NEDD4 antibody

Antibody type

Polyclonal

Description

KD/KO validated NEDD4 antibody (Cat. #21698-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, monkey and has been validated for the following applications: IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Ceftriaxone Modulates Ubiquitination of α-Amino-3-Hydroxy-5-Methyl-4-Isoxazole Propionic Acid Receptors to Improve Long-Term Potentiation Impairment Induced by Exogenous β-Amyloid in a Glutamate Transporter-1 Dependent Manner.

Jiang R, Li L, Wang B, Liu L, Liu L, Xian X, Li W
Molecular neurobiology 2024 Sep;61(9):7181-7194

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ECHDC2 inhibits the proliferation of gastric cancer cells by binding with NEDD4 to degrade MCCC2 and reduce aerobic glycolysis.

He J, Yi J, Ji L, Dai L, Chen Y, Xue W
Molecular medicine (Cambridge, Mass.) 2024 May 23;30(1):69

---

Mecp2 fine-tunes quiescence exit by targeting nuclear receptors.

Yang J, Zou S, Qiu Z, Lai M, Long Q, Chen H, Lai PL, Zhang S, Rao Z, Xie X, Gong Y, Liu A, Li M, Bai X
eLife 2024 May 15;12

---

Inhibition of ADAM9 promotes the selective degradation of KRAS and sensitizes pancreatic cancers to chemotherapy.

Huang YK, Cheng WC, Kuo TT, Yang JC, Wu YC, Wu HH, Lo CC, Hsieh CY, Wong SC, Lu CH, Wu WL, Liu SJ, Li YC, Lin CC, Shen CN, Hung MC, Lin JT, Yeh CC, Sher YP
Nature cancer 2024 Mar;5(3):400-419

---

Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK.

Li Y, Munoz-Mayorga D, Nie Y, Kang N, Tao Y, Lagerwall J, Pernaci C, Curtin G, Coufal NG, Mertens J, Shi L, Chen X
Cell metabolism 2024 Jun 4;36(6):1351-1370.e8

---

PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer.

Xu L, Xiang W, Yang J, Gao J, Wang X, Meng L, Ye K, Zhao XH, Zhang XD, Jin L, Ye Y
Journal of experimental & clinical cancer research : CR 2024 Jan 11;43(1):17

---

Inhibition of RIPK1 alleviating vascular smooth muscle cells osteogenic transdifferentiation via Runx2.

Li Y, Jie W, Qi Y, Mo M, Lian Y, Yin L, Huang H
iScience 2024 Feb 16;27(2):108766

---

The matrix protein of lyssavirus hijacks autophagosome for efficient egress by recruiting NEDD4 through its PPxY motif.

Yuan Y, Fang A, Wang Z, Chen H, Fu ZF, Zhou M, Zhao L
Autophagy 2024 Aug;20(8):1723-1740

---

Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer.

Zhong C, Xiong G, Yang H, Du X, Du J, Yao F, Fang W, Deng Y
International journal of biological sciences 2023;19(4):1110-1122

---

Oncogenic Role of the NFATC2/NEDD4/FBP1 Axis in Cholangiocarcinoma.

Zhao W, Zhao J, Li K, Hu Y, Yang D, Tan B, Shi J
Laboratory investigation; a journal of technical methods and pathology 2023 Sep;103(9):100193

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Nedd4-1 regulates human sodium-dependent vitamin C transporter-2 functional expression in neuronal and epithelial cells.

Teafatiller T, Perez O, Kitazawa M, Agrawal A, Subramanian VS
The Journal of nutritional biochemistry 2023 Oct;120:109413

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Hepatocytic lipocalin-2 controls HDL metabolism and atherosclerosis via Nedd4-1-SR-BI axis in mice.

Hu S, Zhu Y, Zhao X, Li R, Shao G, Gong D, Hu C, Liu H, Xu K, Liu C, Xu M, Zhao Z, Li T, Hu Z, Shao M, Liu J, Li X, Wu H, Li J, Xu Y
Developmental cell 2023 Nov 6;58(21):2326-2337.e5

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FGF21 protects against doxorubicin-induced cardiotoxicity by inhibiting connexin 43 ubiquitination.

Huang Y, Wei C, Li P, Shao Y, Wang M, Wang F, Niu G, Sun K, Zhang Q, Gou Z, Yan X
Free radical biology & medicine 2023 Nov 1;208:748-758

---

Xinbao Pill attenuated chronic heart failure by suppressing the ubiquitination of β-adrenergic receptors.

Tian X, Huang Z, Wang Y, Qi X, Wang D, Liu Z, Cheng Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 Jul;115:154830

---

CFDP1 promotes hepatocellular carcinoma progression through activating NEDD4/PTEN/PI3K/AKT signaling pathway.

Zhou Y, Qiu J, Liu S, Wang P, Ma D, Zhang G, Cao Y, Hu L, Wang Z, Wu J, Jiang C
Cancer medicine 2023 Jan;12(1):425-444

---

hCINAP regulates the differentiation of embryonic stem cells by regulating NEDD4 liquid-liquid phase-separation-mediated YAP1 activation.

Zhuge R, Wang C, Wang J, Yu S, Liao L, Zheng X
Cell reports 2023 Jan 31;42(1):111935

---

NEDD4 ameliorates myocardial reperfusion injury by preventing macrophages pyroptosis.

Sun W, Lu H, Cui S, Zhao S, Yu H, Song H, Ruan Q, Zhang Y, Chu Y, Dong S
Cell communication and signaling : CCS 2023 Feb 2;21(1):29

---

High glucose environment induces NEDD4 deficiency that impairs angiogenesis and diabetic wound healing.

Guo Y, Wang Y, Liu H, Jiang X, Lei S
Journal of dermatological science 2023 Dec;112(3):148-157

---

14-3-3σ-NEDD4L axis promotes ubiquitination and degradation of HIF-1α in colorectal cancer.

Liu S, Guo R, Xu H, Yang J, Luo H, Yeung SJ, Li K, Lee MH, Yang R
Cell reports 2023 Aug 29;42(8):112870

---

PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.

Wang X, Wang R, Jiang K, Zhu M, Guo L, Wu C
Theranostics 2022;12(6):2613-2630

---

Identification of Marek's disease virus pUL56 homologue and analysis of critical amino acid stretches indispensable for its intracellular localization.

Zhang J, Xu B, Ma D, Song J, Liu X, Wei P, Huang T
Virus research 2022 May;313:198741

---

NEDD4 attenuates phosgene-induced acute lung injury through the inhibition of Notch1 activation.

Shao Y, Jiang Z, He D, Shen J
Journal of cellular and molecular medicine 2022 May;26(10):2831-2840

---

Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity Via Inhibiting USP33-Mediated ADRB2 Deubiquitination.

Niu Y, Jiang H, Yin H, Wang F, Hu R, Hu X, Peng B, Shu Y, Li Z, Chen S, Guo F
Diabetes 2022 May 1;71(5):921-933

---

Mesenchymal stem cells-derived small extracellular vesicles alleviate diabetic retinopathy by delivering NEDD4.

Sun F, Sun Y, Zhu J, Wang X, Ji C, Zhang J, Chen S, Yu Y, Xu W, Qian H
Stem cell research & therapy 2022 Jul 15;13(1):293

---

Samotolisib Attenuates Acute Liver Injury Through Inhibiting Caspase-11-Mediated Pyroptosis Via Regulating E3 Ubiquitin Ligase Nedd4.

Zhao YY, Wu DM, He M, Zhang F, Zhang T, Liu T, Li J, Li L, Xu Y
Frontiers in pharmacology 2021;12:726198

---

Distinct and Overlapping Roles of Hippo Effectors YAP and TAZ During Human and Mouse Hepatocarcinogenesis.

Wang H, Wang J, Zhang S, Jia J, Liu X, Zhang J, Wang P, Song X, Che L, Liu K, Ribback S, Cigliano A, Evert M, Wu H, Calvisi DF, Zeng Y, Chen X
Cellular and molecular gastroenterology and hepatology 2021;11(4):1095-1117

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Tumor-derived exosomal miR-19b-3p facilitates M2 macrophage polarization and exosomal LINC00273 secretion to promote lung adenocarcinoma metastasis via Hippo pathway.

Chen J, Zhang K, Zhi Y, Wu Y, Chen B, Bai J, Wang X
Clinical and translational medicine 2021 Sep;11(9):e478

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Human cytomegalovirus UL42 protein inhibits the degradation of glycoprotein B through inhibition of Nedd4 family ubiquitin E3 ligases.

Koshizuka T, Kondo H, Kato H, Takahashi K
Microbiology and immunology 2021 Nov;65(11):472-480

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HECT E3 Ubiquitin Ligase Nedd4 Is Required for Antifungal Innate Immunity.

Nuro-Gyina PK, Tang N, Guo H, Yan C, Zeng Q, Waldschmidt TJ, Zhang J
Journal of immunology (Baltimore, Md. : 1950) 2021 Aug 1;207(3):868-877

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Identification of a new natural biflavonoids against breast cancer cells induced ferroptosis via the mitochondrial pathway.

Xie Y, Zhou X, Li J, Yao XC, Liu WL, Kang FH, Zou ZX, Xu KP, Xu PS, Tan GS
Bioorganic chemistry 2021 Apr;109:104744

---

JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling.

Ren Y, Chen D, Zhai Z, Chen J, Li A, Liang Y, Zhou J
Cell death discovery 2021 Apr 19;7(1):85

---

Ubiquitin ligase CHIP regulates OTUD3 stability and suppresses tumour metastasis in lung cancer.

Zhang P, Li C, Li H, Yuan L, Dai H, Peng Z, Deng Z, Chang Z, Cui CP, Zhang L
Cell death and differentiation 2020 Nov;27(11):3177-3195

---

Multivesicular body sorting and the exosomal pathway are required for the release of rat hepatitis E virus from infected cells.

Primadharsini PP, Nagashima S, Takahashi M, Kobayashi T, Nishiyama T, Nishizawa T, Yasuda J, Mulyanto, Okamoto H
Virus research 2020 Mar;278:197868

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Promotion of liver growth by CAR is accompanied by Akt pathway activation and FoxM1-Nedd4-mediated repression of PTEN.

Yarushkin AA, Mazin ME, Pustylnyak YA, Prokopyeva EA, Pustylnyak VO
Archives of biochemistry and biophysics 2019 Sep 15;672:108065

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NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.

Xu J, Sheng Z, Li F, Wang S, Yuan Y, Wang M, Yu Z
Experimental cell research 2019 Oct 1;383(1):111505

---

YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein.

Shen X, Zhong J, Yu P, Zhao Q, Huang T
Biochemical and biophysical research communications 2019 Feb 5;509(2):448-454

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N-myc downstream-regulated gene 1 inhibits the proliferation of colorectal cancer through emulative antagonizing NEDD4-mediated ubiquitylation of p21.

Zhang S, Yu C, Yang X, Hong H, Lu J, Hu W, Hao X, Li S, Aikemu B, Yang G, He Z, Zhang L, Xue P, Cai Z, Ma J, Zang L, Feng B, Yuan F, Sun J, Zheng M
Journal of experimental & clinical cancer research : CR 2019 Dec 12;38(1):490

---

Dynamic recruitment of ubiquitin to mutant huntingtin inclusion bodies.

Juenemann K, Jansen AHP, van Riel L, Merkx R, Mulder MPC, An H, Statsyuk A, Kirstein J, Ovaa H, Reits EA
Scientific reports 2018 Jan 23;8(1):1405

---

Inhibition of ubiquitin proteasome function prevents monocrotaline-induced pulmonary arterial remodeling.

Zhu Y, Wu Y, Shi W, Wang J, Yan X, Wang Q, Liu Y, Yang L, Gao L, Li M
Life sciences 2017 Mar 15;173:36-42

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Phosphatase and tensin homolog deleted on chromosome 10 degradation induced by NEDD4 promotes acquired erlotinib resistance in non-small-cell lung cancer.

Sun H, Ma H, Wang J, Xia L, Zhu G, Wang Z, Sun J, Chen Z
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Jul;39(7):1010428317709639

---

Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis.

Li S, Mi L, Yu L, Yu Q, Liu T, Wang GX, Zhao XY, Wu J, Lin JD
Proceedings of the National Academy of Sciences of the United States of America 2017 Aug 22;114(34):E7111-E7120

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O-GlcNAc regulates NEDD4-1 stability via caspase-mediated pathway.

Jiang K, Bai B, Ta Y, Zhang T, Xiao Z, Wang PG, Zhang L
Biochemical and biophysical research communications 2016 Mar 18;471(4):539-44

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

C6 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP(NEDD4 antibody) at dilution of 1:300

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The NEDD4 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human NEDD4. This antibody recognizes human,mouse,rat, monkey antigen. The NEDD4 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

21698-1-AP.pdf

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