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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- TA319441 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-NEDD4 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-NEDD4 antibody
- Reactivity
- Horse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- NEDD4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.64 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-Nedd4 antibody shows detection of a 115 kDa band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates.? The blot was blocked with B501-0500 5% BLOTTO overnight at 4°C.? Primary antibody was used at a 1:350 dilution in 5% BLOTTO followed by reaction with a 1:20,000 dilution of HRP goat anti-rabbit IgG in #MB-070 Blocking Buffer for Fluorescent Western Blotting.? ECL was used for detection.
- Validation comment
- WB
