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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52847PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52847PU-N, RRID:AB_11147035
- Product name
- anti NEDD4 (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 977~1007 amino acids from the C-terminal region of human NEDD4
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of NEDD4 Antibody (C-term) Cat.-No AP52847PU-NÂ in mouse NIH-3T3 cell line lysates (35ug/lane). NEDD4 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue reacted with NEDD4 Antibody (C-term) Cat.-No AP52847PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of the NEDD4 Antibody (C-term) for Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H460 cells using NEDD4 Antibody (C-term) Cat.-No AP52847PU-NÂ (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
