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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09405PU-S - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-NEDD4 Antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-NEDD4 Antibody, Aff - Purified
- Reactivity
- Human, Canine, Rabbit
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25ul
- Concentration
- 0.64 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-Nedd4 antibody shows detection of a 115 kDa band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates. The blot was blocked with 5% BLOTTO overnight at 4°C. Primary antibody was used at a 1:350 dilution in 5% BLOTTO followed by reaction with a 1:20,000 dilution of HRP goat anti-rabbit IgG in Blocking Buffer for Fluorescent Western Blotting. ECL was used for detection.
- Validation comment
- WB
