LS-C483076

antibody from LSBio

Targeting: WDFY3 ALFY, KIAA0993, ZFYVE25

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C483076

Provider

LSBio

Product name

WDFY3 / ALFY Antibody (aa2750-2800) LS-C483076

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to WDFY3 (2750-2800). IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.