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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- A301-869A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WDFY3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human WDFY3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-WDFY3 antibody A301-869A (lot A301-869A-2) used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. WDFY3 was also immunoprecipitated by a previous lot (lot A301-869A-1) of this antibody and by rabbit anti-WDFY3 antibody BL7342, which recognizes a downstream epitope. For blotting immunoprecipitated WDFY3, the ReliaBLOT® Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human WDFY3 by immunohistochemistry. Sample: FFPE section of human glioblastoma. Antibody: Affinity purified rabbit anti-WDFY3 (Cat. No. A301-869A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.
