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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [2]
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- Product number
- MAB8200 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Ghrelin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse and human Ghrelin/Obestatin prepropeptide in ELISA. Detects human Ghrelin peptides (acylated and de-acylated) in ELISA.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q9EQX0- Isotype
- IgG
- Antibody clone number
- 883622
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Neurogenin3 phosphorylation controls reprogramming efficiency of pancreatic ductal organoids into endocrine cells.
Neuropeptide Y expression marks partially differentiated β cells in mice and humans.
Azzarelli R, Rulands S, Nestorowa S, Davies J, Campinoti S, Gillotin S, Bonfanti P, Göttgens B, Huch M, Simons B, Philpott A
Scientific reports 2018 Oct 18;8(1):15374
Scientific reports 2018 Oct 18;8(1):15374
Neuropeptide Y expression marks partially differentiated β cells in mice and humans.
Rodnoi P, Rajkumar M, Moin ASM, Georgia SK, Butler AE, Dhawan S
JCI insight 2017 Jun 15;2(12)
JCI insight 2017 Jun 15;2(12)
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Ghrelin in Mouse Stomach. Ghrelin was detected in immersion fixed frozen sections of mouse stomach using Rat Anti-Human/Mouse Ghrelin Monoclonal Antibody (Catalog # MAB8200) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining is shown at both 60x magnification (upper panel) and 20x magnification (lower panel). Staining was localized to cytoplasm and gastric endocrine cells, respectively. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Ghrelin in Human Stomach. Ghrelin was detected in immersion fixed paraffin-embedded sections of human stomach using Rat Anti-Human/Mouse Ghrelin Monoclonal Antibody (Catalog # MAB8200) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
