H00051738-M01

antibody from Abnova Corporation

Targeting: GHRL ghrelin, MTLRP, obestatin

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00051738-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00051738-M01, RRID:AB_565766

Product name

GHRL monoclonal antibody (M01), clone 2F4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant GHRL.

Antigen sequence

MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRV
QQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAED
EMEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDI
LWEEAKEAPADK


Isotype

IgG

Antibody clone number

2F4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Mapping of ghrelin gene expression and cell distribution in the stomach of morbidly obese patients--a possible guide for efficient sleeve gastrectomy construction.

Goitein D, Lederfein D, Tzioni R, Berkenstadt H, Venturero M, Rubin M
Obesity surgery 2012 Apr;22(4):617-22

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GHRL monoclonal antibody (M01), clone 2F4. Western Blot analysis of GHRL expression in HeLa.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GHRL is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to GHRL on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol