PA1-1070

antibody from Invitrogen Antibodies

Targeting: GHRL ghrelin, MTLRP, obestatin

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-1070

Provider

Invitrogen Antibodies

Product name

Ghrelin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-1070 detects Obestatin in mouse and rat samples. This product does not detect endogenous levels of the hormone in rat stomach. PA1-1070 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~2.5 kDa protein representing synthetic Obestatin. The PA1-1070 immunogen is a synthetic peptide corresponding to residues F(1) N A P F D V G I K L S G A Q Y Q Q H G R A L(23) of the rat Obestatin gene. This sequence is conserved in mouse and only one amino acid residue difference in human. This peptide (Cat. # PEP-290) is available for use in neutralization and control experiments.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Study of the Ghrelin/LEAP-2 Ratio in Humans and Rats during Different Phases of Pregnancy.

Garcés MF, Buell-Acosta JD, Ángel-Müller E, Parada-Baños AJ, Acosta-Alvarez J, Saavedra-López HF, Franco-Vega R, Maldonado-Acosta LM, Escobar-Cordoba F, Vásquez-Romero K, Lacunza E, Caminos-Cepeda SA, Nogueiras R, Diéguez C, Ruiz-Parra AI, Caminos JE
International journal of molecular sciences 2022 Aug 23;23(17)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GHRL was performed using 70% confluent log phase MKN-45 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ghrelin Rabbit Polyclonal Antibody (Product # PA1-1070) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunolocalization of LEAP-2 and Ghrelin protein in human tissues. ( A ). LEAP-2 protein is immunolocated in liver tissue (10x). ( B ). Ghrelin protein is immunolocated in human gastric mucosa (filled arrow in ( B ) (4x). ( C , D ) show immunostaining of LEAP-2 ( C ) and Ghrelin ( D ), respectively in cytotrophoblasts (filled arrow) (40x) and syncytiotrophoblasts (arrows) (40x) sections of human term placentas.