Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [7]
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Validation data
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- Product number
- NBP1-89773 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89773, RRID:AB_11038386
- Product name
- Rabbit Polyclonal Ghrelin/Obestatin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Ghrelin/Obestatin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Ghrelin/Obestatin Antibody [NBP1-89773] - Analysis in control (vector only transfected HEK293T lysate) and GHRL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells and extracellular material in glandular lumina.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human stomach shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Analysis in human stomach and liver tissues. Corresponding Ghrelin/Obestatin RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human colon shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human duodenum shows weak cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human liver shows no positivity in hepatocytes as expected.