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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- PA1-31074 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAM17 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX13535-PEP).. Expected to cross-react with Chinese Hamster due to sequence homology. Detects a band of approximately 125-130 kDa (predicted molecular weight: 95 or when glycosylated 130 kDa).
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TACE/ADAM17 in Hela lysate (RIPA buffer 35 µg total protein per lane) using a TACE/ADAM17 polyclonal antibody (Product # PA1-31074) at a dilution of 0.2 µg/mL following incubation for 1 hour and detected using chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ADAM17 was performed by loading 35 µg (in RIPA buffer) of Daudi (A), Jurkat (B) and HeLa (C) lysates. Proteins were transferred to a membrane and probed with a ADAM17 Polyclonal Antibody (Product # PA1-31074) at a dilution of 0.3 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ADAM17 was performed by loading 35 µg (in RIPA buffer) of Daudi (A), Jurkat (B) and HeLa (C) lysates. Proteins were transferred to a membrane and probed with a ADAM17 Polyclonal Antibody (Product # PA1-31074) at a dilution of 0.3 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ADAM17 was performed by loading 35 µg (in RIPA buffer) of human testis lysates. Proteins were transferred to a membrane and probed with a ADAM17 Polyclonal Antibody (Product # PA1-31074) at a dilution of 0.2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ADAM17 in human skin tissue using ADAM17 Polyclonal Antibody (Product # PA1-31074) at a dilution of 3.75 µg/mL. Antigen retrieval : citrate buffer pH 6.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ADAM17 in human thyroid tissue using ADAM17 Polyclonal Antibody (Product # PA1-31074) at a dilution of 3.75 µg/mL. Antigen retrieval : citrate buffer pH 6.
