PA1-31074

antibody from Invitrogen Antibodies

Targeting: ADAM17 CD156B, cSVP, TACE

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-31074

Provider

Invitrogen Antibodies

Product name

ADAM17 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX13535-PEP)..

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TACE/ADAM17 in Hela lysate (RIPA buffer 35 µg total protein per lane) using a TACE/ADAM17 polyclonal antibody (Product # PA1-31074) at a dilution of 0.2 µg/mL following incubation for 1 hour and detected using chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ADAM17 was performed by loading 35 µg (in RIPA buffer) of Daudi (A), Jurkat (B) and HeLa (C) lysates. Proteins were transferred to a membrane and probed with a ADAM17 Polyclonal Antibody (Product # PA1-31074) at a dilution of 0.3 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ADAM17 was performed by loading 35 µg (in RIPA buffer) of Daudi (A), Jurkat (B) and HeLa (C) lysates. Proteins were transferred to a membrane and probed with a ADAM17 Polyclonal Antibody (Product # PA1-31074) at a dilution of 0.3 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ADAM17 was performed by loading 35 µg (in RIPA buffer) of human testis lysates. Proteins were transferred to a membrane and probed with a ADAM17 Polyclonal Antibody (Product # PA1-31074) at a dilution of 0.2 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ADAM17 in human skin tissue using ADAM17 Polyclonal Antibody (Product # PA1-31074) at a dilution of 3.75 µg/mL. Antigen retrieval : citrate buffer pH 6.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ADAM17 in human thyroid tissue using ADAM17 Polyclonal Antibody (Product # PA1-31074) at a dilution of 3.75 µg/mL. Antigen retrieval : citrate buffer pH 6.