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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA1-31481 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-ADAM17 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-31481 detects TACE from human, mouse, rat samples. PA1-31481 has been successfully used in Western blot applications. The PA1-31481 immunogen is: Peptide sequence - amino acids 807 - 823 of human TACE. (This sequence differs from mouse and rat TACE by one amino acid) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TACE/ADAM17 in HeLa (1, 3) and Jurkat (2, 4) whole cell lysate in the absence (1, 2) or presence (3, 4) of blocking peptide using a TACE/ADAM17 polyclonal antibody (Product # PA1-31481).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of TACE/ADAM17 in HeLa cells using a TACE/ADAM17 polyclonal antibody (Product # PA1-31481) at a dilution of 10µg/mL.
