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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [4]
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- Product number
- NBP1-77044 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77044, RRID:AB_11037985
- Product name
- Rabbit Polyclonal TACE/ADAM17 Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE/ADAM17. Human TACE/ADAM17 has 2 isoforms, including isoform A (824aa, 93kD), isoform B (694aa, 78.5kD). This antibody detects only isoform A. Mouse TACE/ADAM17 has 2 isoforms, long isoform (827aa, 93.1kD) and short isoform (655aa, 73.9kD). Rat TACE/ADAM17 has only one isoform identified so far (827aa, 93kD).
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Tobacco smoke-induced lung cell proliferation mediated by tumor necrosis factor alpha-converting enzyme and amphiregulin.
Lemjabbar H, Li D, Gallup M, Sidhu S, Drori E, Basbaum C
The Journal of biological chemistry 2003 Jul 11;278(28):26202-7
The Journal of biological chemistry 2003 Jul 11;278(28):26202-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - HeLa whole cell lysate. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - Analysis of TACE in HeLa (A, D), Jurkat (B, E) and Raji (C,F) cell lysate in (A-C) the absence or (D-F) the presence of blocking peptide with TACE antibody at 0.5 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - IAV via Protein Expression Profile in Cells Lines. Antibodies: TACE and GAPDH. Secondary: Goat anti-rabbit IgG HRP conjugate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TACE KO cell lysates. Antibodies: TACE/ADAM17 NBP1-77044 (0.25 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
