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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-19872 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAM17 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is Hela cell lysate. A 17 amino acid peptide near the carboxy terminus of human TACE. The PA5-19872 immunogen is located within the last 50 amino acids of TACE. Predicted molecular ~ 93kD. In Western blot applications, this antibody has been observed to detect a band at: 93-125kD (Post-modification: 9 N-linked glycosylation). Predicted species reactivity based on immunogen sequence: mouse (94.1%).
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references MAGI-1 PDZ2 Domain Blockade Averts Adenovirus Infection via Enhanced Proteolysis of the Apical Coxsackievirus and Adenovirus Receptor.
Alghamri MS, Sharma P, Williamson TL, Readler JM, Yan R, Rider SD Jr, Hostetler HA, Cool DR, Kolawole AO, Excoffon KJDA
Journal of virology 2021 Jun 10;95(13):e0004621
Journal of virology 2021 Jun 10;95(13):e0004621
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HeLa and Jurkat whole cell lysate in the absence or presence of blocking peptide (Product # PEP-0008) using a CD156b/ADAM17/TACE polyclonal antibody (Product # PA5-19872) at 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ADAM17 in HeLa WT or TACE KO cells. Lysates (10 µg) were loaded onto SDS-PAGE and blots were probed with ADAM17 Polyclonal Antibody (Product # PA5-19872) diluted to 0.25 µg/mL and anti-beta actin diluted to 1 µg/mL. 1 h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation of TACE in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: ADAM17 Polyclonal Antibody (Product # PA5-19872) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lanes: HeLa (A,D), Jurkat (B, E), Raji (C,F) in the absence (A-C) or presence (E-F) of blocking peptide.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a CD156b/ADAM17/TACE polyclonal antibody (Product # PA5-19872) at a 10 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of HeLa cells using ADAM17 Polyclonal Antibody (Product # PA5-19872) at 10 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE with ADAM17 Polyclonal Antibody (Product # PA5-19872) at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HeLa cells using a CD156b/ADAM17/TACE polyclonal antibody (Product # PA5-19872) at a 10 µg/mL dilution.
