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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB100-1012 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1012, RRID:AB_2223547
- Product name
- Goat Polyclonal TACE/ADAM17 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise isoform 1 (represented by NP_003174) and not isoform 2 (represented by NP_068604).
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells.
Black RA, Rauch CT, Kozlosky CJ, Peschon JJ, Slack JL, Wolfson MF, Castner BJ, Stocking KL, Reddy P, Srinivasan S, Nelson N, Boiani N, Schooley KA, Gerhart M, Davis R, Fitzner JN, Johnson RS, Paxton RJ, March CJ, Cerretti DP
Nature 1997 Feb 20;385(6618):729-33
Nature 1997 Feb 20;385(6618):729-33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TACE/ADAM17 Antibody [NB100-1012] - staining (0.2ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TACE/ADAM17 Antibody [NB100-1012] - (0.3ug/ml) of Daudi (A), Jurkat (B) and HeLa (C) lysates (35ug total protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NB100-1012] - (3.75ug/ml) staining of Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NB100-1012] - (3.75ug/ml) staining of Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
