Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF9301 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TACE/ADAM17 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human TACE/ADAM17 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P78536
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dysregulation of the ADAM17/Notch signalling pathways in endometriosis: from oxidative stress to fibrosis.
ADAM-12 as a diagnostic marker for the proliferation, migration and invasion in patients with small cell lung cancer.
González-Foruria I, Santulli P, Chouzenoux S, Carmona F, Chapron C, Batteux F
Molecular human reproduction 2017 Jul 1;23(7):488-499
Molecular human reproduction 2017 Jul 1;23(7):488-499
ADAM-12 as a diagnostic marker for the proliferation, migration and invasion in patients with small cell lung cancer.
Shao S, Li Z, Gao W, Yu G, Liu D, Pan F
PloS one 2014;9(1):e85936
PloS one 2014;9(1):e85936
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TACE/ADAM17 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACE/ADAM17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9301) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TACE/ADAM17 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TACE/ADAM17 in Human Colon. TACE/ADAM17 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human TACE/ADAM17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9301) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.