bs-3738R

antibody from Bioss

Targeting: CRKL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

bs-3738R

Provider

Bioss

Proper citation

Bioss Cat#bs-3738R, RRID:AB_10857331

Product name

Crkl(Tyr251) Polyclonal Antibody

Antibody type

Polyclonal

Description

KLH conjugated synthetic phosphopeptide derived from human Crkl around the phosphorylation site of Tyr251 [CA(p-Y)D]

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100ul

Concentration

1ug/ul

Storage

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Western blot

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

L1 mouse embryo lysates L2 mouse intestine lysates probed with Anti phospho-Crkl(Tyr251) Polyclonal Antibody, Unconjugated (bs-3738R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 33kD. Observed band size:33kD.

Sample type

Mouse

Other comments

Embryo

Immunohistochemistry

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Rabbit Anti phospho-Crkl(Tyr251) Polyclonal Antibody, Unconjugated (bs-3738R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Sample type

Human

Other comments

Colon