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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6084 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6084, RRID:AB_1574102
- Product name
- CRKL polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of CRKL.
- Antigen sequence
C-KIFDPQNPDENE- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Active (p)CrkL is overexpressed in human malignancies: potential role as a surrogate parameter for therapeutic tyrosine kinase inhibition.
Singer CF, Hudelist G, Lamm W, Mueller R, Handl C, Kubista E, Czerwenka K
Oncology reports 2006 Feb;15(2):353-9
Oncology reports 2006 Feb;15(2):353-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CRKL polyclonal antibody (Cat # PAB6084) staining (0.01 ug/mL) of K-562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CRKL polyclonal antibody (Cat # PAB6084) (3.8 ug/mL) staining of paraffin embedded human thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
