711850

antibody from Invitrogen Antibodies

Targeting: CRKL

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711850

Provider

Invitrogen Antibodies

Product name

Phospho-CrkL (Tyr207) Recombinant Polyclonal Antibody (4HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Cat, Horse, Mouse.

Antibody clone number

4HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and K-562 treated with Imatinib (5 µM for 24 hrs) (Lane 2). The blots were probed with Phospho-CrkL (Tyr207) Recombinant Rabbit Polyclonal Antibody (Product # 711850, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 39 kDa band corresponding to Phospho-CrkL (Tyr207) was observed only in untreated K-562, while treatment with Imatinib downregulated its expression. Antibody specificity was demonstrated by competition with the Phospho-CrkL (Tyr207) peptide, which resulted in loss of signal. No competition was observed with the Non-phospho peptide.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1), K-562 treated with Imatinib (0.1 µM for 24 hrs) (Lane 2), K-562 treated with Imatinib (1 µM for 24 hrs) (Lane 3) and K-562 treated with Imatinib (5 µM for 24 hrs) (Lane 4). The blot was probed with Phospho-CrkL (Tyr207) Recombinant Rabbit Polyclonal Antibody (Product # 711850, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 39 kDa band corresponding to Phospho-CrkL (Tyr207) was observed in untreated K-562 and subsequent decrease in expression was detected in K-562 treated with increasing concentrations of Imatinib.