Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
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Validation data
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- Product number
- 711850 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-CrkL (Tyr207) Recombinant Polyclonal Antibody (4HCLC)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 4HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and K-562 treated with Imatinib (5 µM for 24 hrs) (Lane 2). The blots were probed with Phospho-CrkL (Tyr207) Recombinant Rabbit Polyclonal Antibody (Product # 711850, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 39 kDa band corresponding to Phospho-CrkL (Tyr207) was observed only in untreated K-562, while treatment with Imatinib downregulated its expression. Antibody specificity was demonstrated by competition with the Phospho-CrkL (Tyr207) peptide, which resulted in loss of signal. No competition was observed with the Non-phospho peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1), K-562 treated with Imatinib (0.1 µM for 24 hrs) (Lane 2), K-562 treated with Imatinib (1 µM for 24 hrs) (Lane 3) and K-562 treated with Imatinib (5 µM for 24 hrs) (Lane 4). The blot was probed with Phospho-CrkL (Tyr207) Recombinant Rabbit Polyclonal Antibody (Product # 711850, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 39 kDa band corresponding to Phospho-CrkL (Tyr207) was observed in untreated K-562 and subsequent decrease in expression was detected in K-562 treated with increasing concentrations of Imatinib.