H00001399-M03

antibody from Novus Biologicals

Targeting: CRKL

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

H00001399-M03

Provider

Novus Biologicals

Proper citation

Novus Cat#H00001399-M03, RRID:AB_828764

Product name

Mouse Monoclonal CrkL Antibody

Antibody type

Monoclonal

Description

IgG purified. CRKL (4B5)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Analysis of protein-protein interactions in cross-talk pathways reveals CRKL protein as a novel prognostic marker in hepatocellular carcinoma.

Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY
Molecular & cellular proteomics : MCP 2013 May;12(5):1335-49

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PI3K links NKG2D signaling to a CrkL pathway involved in natural killer cell adhesion, polarity, and granule secretion.

Segovis CM, Schoon RA, Dick CJ, Nacusi LP, Leibson PJ, Billadeau DD
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 1;182(11):6933-42

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CrkL Antibody (4B5) [H00001399-M03] - Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL Validated Chimera RNAi or non-transfected control. Blot probed with H00001399-M03. GAPDH (36.1 kDa) used as loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CrkL Antibody (4B5) [H00001399-M03] - Analysis of CRKL expression in transfected 293T cell line by CRKL monoclonal antibody (M03), clone 4B5. Lane 1: CRKL transfected lysatE (33.8 KDa). Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: CrkL Antibody (4B5) [H00001399-M03] - Detection limit for recombinant GST tagged CRKL is approximately 0.3ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03] - Analysis of protein-protein interactions between GAB1 and CRKL. HeLa cells were stained with anti-GAB1 rabbit purified polyclonal 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction com

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03] - Analysis of protein-protein interactions between PTK2 and CRKL. Huh7 cells were stained with anti-PTK2 rabbit purified polyclonal 1:600 and anti-CRKL mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction com

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03] - Analysis of monoclonal antibody to CRKL on HeLa cell. Antibody concentration 10 ug/ml