Ab097773

antibody from Aladdin Scientific

Targeting: CRKL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab097773

Provider

Aladdin Scientific

Product name

Recombinant CrkL Antibody

Antibody type

Recombinant

Description

Rabbit anti Human CrkL Antibody, Recombinant, could be used for WB, IF, ICC and so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200Protein FunctionMay mediate the transduction of intracellular signals.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 11-25


Vial size

100_l,10_l,1ml,50_l

Concentration

0,5 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant CrkL Antibody (Ab097773) - Western Blot All lanes: Recombinant CrkL Antibody (Ab097773) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 36 kDa Exposure time: 78.7 s

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant CrkL Antibody (Ab097773) - Western Blot All lanes: Recombinant CrkL Antibody (Ab097773) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 36 kDa Exposure time: 100.0 s

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant CrkL Antibody (Ab097773) - IF/ICC Immunocytochemistry analysis of CrkL (green) in K562 using Recombinant CrkL Antibody (Ab097773), and cells were counterstained with DAPI (blue).