Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB300-884 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-884, RRID:AB_10001482
- Product name
- Goat Polyclonal CrkL Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Active (p)CrkL is overexpressed in human malignancies: potential role as a surrogate parameter for therapeutic tyrosine kinase inhibition.
Singer CF, Hudelist G, Lamm W, Mueller R, Handl C, Kubista E, Czerwenka K
Oncology reports 2006 Feb;15(2):353-9
Oncology reports 2006 Feb;15(2):353-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CrkL Antibody [NB300-884] - K562 cell lysate (35 ug protein in RIPA buffer). Primary antibody at 0.01 ug/ml, 1 hr incubation. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CrkL Antibody [NB300-884] - Paraffin embedded human thymus. Antibody at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.