Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN1504663 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Antigen Affinity Chromatography
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mL
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western Blot Analysis/Peptide Blocking: K562 cell lysate was resolved by SDS-PAGE, transferred to PVDF, and probed with anti-phospho-CrkL (Tyr207) at a 1/1,000 dilution. Lane 1 (control) was not peptide blocked. Lanes 2 and 3 were peptide blocked with either non-phosphorylated peptide (Lane 2) or phosphorylated peptide (Lane 3) corresponding to the sequence surrounding Tyr207 of human CrkL. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated CrkL.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western Blot Analysis: K562 cell lysate was resolved by SDS-PAGE, transferred to PVDF, and probed with anti-phospho-CrkL (Tyr207) (1/1,000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated CrkL.
