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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-31907 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Histone H1.4 (Ser35) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Acid-extracted histones from HeLa cell treated Nocodazole. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.93 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Alterations in Protein Kinase A Substrate Specificity as a Potential Cause of Cushing Syndrome.
Bathon K, Weigand I, Vanselow JT, Ronchi CL, Sbiera S, Schlosser A, Fassnacht M, Calebiro D
Endocrinology 2019 Feb 1;160(2):447-459
Endocrinology 2019 Feb 1;160(2):447-459
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of Phospho-Histone H1.4 pSer35 in paraformaldehyde-fixed HeLa cells using a Phospho-Histone H1.4 pSer35 polyclonal antibody (Product # PA5-31907) at a 1:500 dilution.
