LS-C804397

antibody from LSBio

Targeting: H1-4 H1.4, H1e, H1F4, H1s-4, HIST1H1E

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C804397

Provider

LSBio

Product name

HIST1H1E Antibody (phospho-Thr17) LS-C804397

Antibody type

Polyclonal

Description

Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of Phospho-Histone H1.3 (T17) +H1.4 (T17) using 293 whole cells lysates

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

1:100 staining mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.