Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- PA5-117472 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H1.4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.06 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Histone H1.4 using Histone H1.4 Polyclonal Antibody (Product # PA5-117472) at 1:500. Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:4,0000 dilution. Predicted band size: 22 kDa. Observed band size: 22 kDa
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of Histone H1.4 in HeLa cells using Histone H1.4 Polyclonal Antibody (Product # PA5-117472) at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Histone H1.4 in paraffin-embedded human colon cancer using Histone H1.4 Polyclonal Antibody (Product # PA5-117472) diluted at 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Histone H1.4 in paraffin-embedded human melanoma using Histone H1.4 Polyclonal Antibody (Product # PA5-117472) diluted at 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP assay of Histone H1.4 in Hela cells (4x10^6). Cells were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8 µg of Histone H1.4 Polyclonal Antibody (Product # PA5-117472) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the Beta-Globin promoter.