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LearnPA5-46942
antibody from Invitrogen Antibodies
Targeting: CCL27
ALP, CTACK, CTAK, ESkine, ILC, PESKY, SCYA27, skinkine
Western blot
Immunohistochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Other assay [2]
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- Product number
- PA5-46942 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCL27 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, less than 2% cross-reactivity with recombinant mouse CCL27/CTACK is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of human CCL27 is shown by decrease in RFU (relative fluorescent units - measure of chemotaxis) with increasing concentrations of CCL27 polyclonal antibody (PA5-46942).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of CCL27 in BaF3 mouse pro‚B cell line transfected with human CCR1. Samples were incubated in CCL27 polyclonal antibody (Product # PA5-46942). Recombinant Human CCL27/CTACK chemoattracts the BaF3 mouse pro‚B cell line transfected with human CCR10/GPR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL27/CTACK (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody. At 55 μg/mL, this antibody will neutralize > 70% of the chemotactic effect.
