HPA038440

antibody from Atlas Antibodies

Targeting: SPAG6 CFAP194, CT141, FAP194, PF16, Repro-SA-1

Provider product page for HPA038440

Western blot

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [19]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: HPA038440 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA038440, RRID:AB_10671742
Product name: Anti-SPAG6
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human SPAG6, Gene description: sperm associated antigen 6, Alternative Gene Names: CT141, pf16, Repro-SA-1, Validated applications: IHC, WB, Uniprot ID: O75602, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.4 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

SPAG6 protein structure - HPA038440 shown in red.

Submitted references Further evidence from DNAH12 supports favorable fertility outcomes of infertile males with dynein axonemal heavy chain gene family variants

Geng H, Wang K, Liang D, Ni X, Yu H, Tang D, Lv M, Wu H, Li K, Shen Q, Gao Y, Xu C, Zhou P, Wei Z, Cao Y, Sha Y, Yang X, He X
iScience 2024;27(7):110366

Novel variants in DNAH6 cause male infertility associated with multiple morphological abnormalities of the sperm flagella (MMAF) and ICSI outcomes

Shao Z, Zhu Y, Gu M, Guo S, Yu H, Li K, Tang D, Xu Y, Lv M
Asian Journal of Andrology 2024;26(1):91-98

Bi-allelic variants in DNAH3 cause male infertility with asthenoteratozoospermia in humans and mice

Meng G, Wang Y, Luo C, Tan Y, Li Y, Tan C, Tu C, Zhang Q, Hu L, Zhang H, Meng L, Liu C, Deng L, Lu G, Lin G, Du J, Tan Y, Sha Y, Wang L, He W
Human Reproduction Open 2024;2024(1)

Association of novel DNAH11 variants with asthenoteratozoospermia lead to male infertility

Guo S, Tang D, Chen Y, Yu H, Gu M, Geng H, Fang J, Wu B, Ruan L, Li K, Xu C, Gao Y, Tan Q, Duan Z, Wu H, Hua R, Guo R, Wei Z, Zhou P, Xu Y, Cao Y, He X, Sha Y, Lv M
Human Genomics 2024;18(1)

Identification of IQCH as a calmodulin-associated protein required for sperm motility in humans

Cavarocchi E, Sayou C, Lorès P, Cazin C, Stouvenel L, El Khouri E, Coutton C, Kherraf Z, Patrat C, Govin J, Thierry-Mieg N, Whitfield M, Ray P, Dulioust E, Touré A
iScience 2023;26(8):107354

Novel FSIP2 Variants Induce Super-Length Mitochondrial Sheath and Asthenoteratozoospermia in Humans

Lv M, Tang D, Yu H, Geng H, Zhou Y, Shao Z, Li K, Gao Y, Guo S, Xu C, Tan Q, Liu C, Guo R, Wu H, Duan Z, Zhang J, Wang G, Hua R, Fu F, Wang K, Xu Y, Zhou P, Wei Z, Zhang F, Cao Y, He X
International Journal of Biological Sciences 2023;19(2):393-411

Novel HYDIN variants associated with male infertility in two Chinese families

Yu H, Shi X, Shao Z, Geng H, Guo S, Li K, Gu M, Xu C, Gao Y, Tan Q, Duan Z, Wu H, Hua R, Guo R, Wei Z, Zhou P, Cao Y, He X, Li L, Zhang X, Lv M
Frontiers in Endocrinology 2023;14

Sperm flagellar 2 (SPEF2) is essential for sperm flagellar assembly in humans

Li D, Yang X, Tu C, Wang W, Meng L, Lu G, Tan Y, Zhang Q, Du J
Asian Journal of Andrology 2022;24(4):359-366

Homozygous SPAG6 variants can induce nonsyndromic asthenoteratozoospermia with severe MMAF

Xu C, Tang D, Shao Z, Geng H, Gao Y, Li K, Tan Q, Wang G, Wang C, Wu H, Li G, Lv M, He X, Cao Y
Reproductive Biology and Endocrinology 2022;20(1)

Bi-allelic variants in DNHD1 cause flagellar axoneme defects and asthenoteratozoospermia in humans and mice

Tan C, Meng L, Lv M, He X, Sha Y, Tang D, Tan Y, Hu T, He W, Tu C, Nie H, Zhang H, Du J, Lu G, Fan L, Cao Y, Lin G, Tan Y
The American Journal of Human Genetics 2022;109(1):157-171

Bi-allelic variants in DNAH10 cause asthenoteratozoospermia and male infertility

Li K, Wang G, Lv M, Wang J, Gao Y, Tang F, Xu C, Yang W, Yu H, Shao Z, Geng H, Tan Q, Shen Q, Tang D, Ni X, Wang T, Song B, Wu H, Huo R, Zhang Z, Xu Y, Zhou P, Tao F, Wei Z, He X, Cao Y
Journal of Assisted Reproduction and Genetics 2021;39(1):251-259

Bi-allelic mutations of DNAH10 cause primary male infertility with asthenoteratozoospermia in humans and mice

Tu C, Cong J, Zhang Q, He X, Zheng R, Yang X, Gao Y, Wu H, Lv M, Gu Y, Lu S, Liu C, Tian S, Meng L, Wang W, Tan C, Nie H, Li D, Zhang H, Gong F, Hu L, Lu G, Xu W, Lin G, Zhang F, Cao Y, Tan Y
The American Journal of Human Genetics 2021;108(8):1466-1477

SPAG6 silencing induces autophagic cell death in SKM‑1 cells via the AMPK/mTOR/ULK1 signaling pathway

Zhang M, Luo J, Luo X, Liu L
Oncology Letters 2020;20(1):551-560

A quantitative super-resolution imaging toolbox for diagnosis of motile ciliopathies

Liu Z, Nguyen Q, Guan Q, Albulescu A, Erdman L, Mahdaviyeh Y, Kang J, Ouyang H, Hegele R, Moraes T, Goldenberg A, Dell S, Mennella V
Science Translational Medicine 2020;12(535)

Bi-allelic Loss-of-function Variants in CFAP58 Cause Flagellar Axoneme and Mitochondrial Sheath Defects and Asthenoteratozoospermia in Humans and Mice

He X, Liu C, Yang X, Lv M, Ni X, Li Q, Cheng H, Liu W, Tian S, Wu H, Gao Y, Yang C, Tan Q, Cong J, Tang D, Zhang J, Song B, Zhong Y, Li H, Zhi W, Mao X, Fu F, Ge L, Shen Q, Zhang M, Saiyin H, Jin L, Xu Y, Zhou P, Wei Z, Zhang F, Cao Y
The American Journal of Human Genetics 2020;107(3):514-526

Identification of DNAH6 mutations in infertile men with multiple morphological abnormalities of the sperm flagella

Tu C, Nie H, Meng L, Yuan S, He W, Luo A, Li H, Li W, Du J, Lu G, Lin G, Tan Y
Scientific Reports 2019;9(1)

Absence of CFAP69 Causes Male Infertility due to Multiple Morphological Abnormalities of the Flagella in Human and Mouse

Dong F, Amiri-Yekta A, Martinez G, Saut A, Tek J, Stouvenel L, Lorès P, Karaouzène T, Thierry-Mieg N, Satre V, Brouillet S, Daneshipour A, Hosseini S, Bonhivers M, Gourabi H, Dulioust E, Arnoult C, Touré A, Ray P, Zhao H, Coutton C
The American Journal of Human Genetics 2018;102(4):636-648

SPAG6 and L1TD1 are transcriptionally regulated by DNA methylation in non-small cell lung cancers

Altenberger C, Heller G, Ziegler B, Tomasich E, Marhold M, Topakian T, Müllauer L, Heffeter P, Lang G, End-Pfützenreuter A, Döme B, Arns B, Klepetko W, Zielinski C, Zöchbauer-Müller S
Molecular Cancer 2017;16(1)

Profiling cancer testis antigens in non-small-cell lung cancer.

Djureinovic D, Hallström BM, Horie M, Mattsson JSM, La Fleur L, Fagerberg L, Brunnström H, Lindskog C, Madjar K, Rahnenführer J, Ekman S, Ståhle E, Koyi H, Brandén E, Edlund K, Hengstler JG, Lambe M, Saito A, Botling J, Pontén F, Uhlén M, Micke P
JCI insight 2016 Jul 7;1(10):e86837

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Recombinant expression validation
Main image
Experimental details: Western blot analysis in control (vector only transfected HEK293T lysate) and SPAG6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406761).
Sample type: Human
Protocol: Protocol

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human fallopian tube and colon tissues using Anti-SPAG6 antibody. Corresponding SPAG6 RNA-seq data are presented for the same tissues.
Sample type: Human
Protocol: Protocol
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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