15199-1-AP

antibody from Proteintech Group

Targeting: TMED10 p23, P24(DELTA), p24d1, p24delta1, TMP21

Provider product page for 15199-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [14]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 15199-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15199-1-AP, RRID:AB_2204321
Product name: TMP21 antibody
Antibody type: Polyclonal
Description: KD/KO validated TMP21 antibody (Cat. #15199-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

TMED10 protein structure - 15199-1-AP shown in red.

Submitted references β-Sitosterol Suppresses Osteoclastogenesis and Alleviates Bone Loss by Targeting Tmed10 to Inhibit RANKL-Induced Hedgehog/MAPK Signaling and ROS.

Huang W, Liu T, Liao J, Lian D, Zeng X, Yao Z, He M, Zhang X, Cai Z
Phytotherapy research : PTR 2026 May;40(5):2491-2513

TMEDs mediate versatile cargo transport in vesicle-dependent unconventional secretion.

Zheng J, Wang H, Sun Y, Chang P, Deng X, Zhang L, Zhu L, Zhu K, Peng D, Deng H, Zhang M, Ge L
The Journal of cell biology 2026 Jan 5;225(1)

Trans-Golgi network-associated noncanonical autophagy depends on the V-ATPase-ATG16L1 axis and mediates IL1B secretion.

Long J, Xie H, Shan H, Chen R, Zhong J, Tang J, Fang D, Wang Y, Liu P, Yin XM, Li M
Autophagy 2026 Apr;22(4):691-708

STING COPII ER Export Trafficking and Signaling Primed by Phosphorylation Switches.

Nan Y, Cui D, Guo J, Ma X, Wang J, Guo L, Li T, Yang M, Huang G, Xu A, Ma W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Sep;12(36):e03660

ER exit sites mediated by the COPII adaptor sec24D selectively recruit lipid raft-preferring proteins for rapid ER export.

Castello-Serrano I, Dagar S, Ippolito R, Levental KR, Levental I
Nature communications 2025 Nov 27;16(1):10694

Coronavirus envelope protein activates TMED10-mediated unconventional secretion of inflammatory factors.

Liu L, Zhang L, Hao X, Wang Y, Zhang X, Ge L, Wang P, Tian B, Zhang M
Nature communications 2024 Oct 8;15(1):8708

A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion.

Sun Y, Tao X, Han Y, Lin X, Tian R, Wang H, Chang P, Sun Q, Ge L, Zhang M
Nature cell biology 2024 Jul;26(7):1077-1092

Active secretion of IL-33 from astrocytes is dependent on TMED10 and promotes central nervous system homeostasis.

Jiao M, Wang C, Tang X, Dai C, Zhang N, Fan A, Qian Z, Liu S, Zhang F, Li B, Xu Y, Tan Z, Gong F, Lu Y, Zheng F
Brain, behavior, and immunity 2024 Jul;119:539-553

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

Li S, Yan R, Xu J, Zhao S, Ma X, Sun Q, Zhang M, Li Y, Liu JG, Chen L, Li S, Xu K, Ge L
Cell research 2022 Feb;32(2):119-138

TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.

Park H, Seo SK, Sim JR, Hwang SJ, Kim YJ, Shin DH, Jang DG, Noh SH, Park PG, Ko SH, Shin MH, Choi JY, Ito Y, Kang CM, Lee JM, Lee MG
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Aug;9(24):e2105320

A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

Zhang M, Liu L, Lin X, Wang Y, Li Y, Guo Q, Li S, Sun Y, Tao X, Zhang D, Lv X, Zheng L, Ge L
Cell 2020 Apr 30;181(3):637-652.e15

The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.

Yang X, Li S, Wu Y, Ge F, Chen Y, Xiong Q
BMC cancer 2020 Apr 15;20(1):312

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Darville LNF, Sokolowski BHA
Proteome science 2018;16:15

A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.

Sun J, Yu S, Zhang X, Capac C, Aligbe O, Daudelin T, Bonder EM, Gao N
Journal of cell science 2017 Jul 1;130(13):2159-2171

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15199-1-AP(TMED10 antibody) at dilution of 1:1000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using TMED10 antibody 15199-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human pancreas using 15199-1-AP(TMED10 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human pancreas using 15199-1-AP(TMED10 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue