Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF7630 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Tmp21/p23 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P49755
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Tmp21/p23 by Western Blot. Western blot shows lysates of mouse pancreas tissue, U-87 MG human glioblastoma/astrocytoma cell line, and human pancreas tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse Tmp21/p23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7630) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tmp21/p23 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human Tmp21/p23 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Tmp21/p23 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse Tmp21/p23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7630) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tmp21/p23 at approximately 23 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Tmp21/p23 in Human Alzheimer's Brain. Tmp21/p23 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Sheep Anti-Human/Mouse Tmp21/p23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7630) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.