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LearnPA1-027A-A488
antibody from Invitrogen Antibodies
Targeting: PPIB
B, CYP-S1, CYPB, OI9, PPIase, SCYLP
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA1-027A-A488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclophilin B Polyclonal Antibody, Alexa Fluor™ 488
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1027AA488 detects cyclophilin B (CyPB) in mouse, rat, canine, and human tissues. The PA1027AA488 immunogen is a synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB. This sequence is almost completely conserved between human, mouse, rat, bovine, and chicken. PA1027AA488 immunizing peptide (Cat. # PEP-058) is available for use in neutralization and control experiments.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references LIFR is a breast cancer metastasis suppressor upstream of the Hippo-YAP pathway and a prognostic marker.
Chen D, Sun Y, Wei Y, Zhang P, Rezaeian AH, Teruya-Feldstein J, Gupta S, Liang H, Lin HK, Hung MC, Ma L
Nature medicine 2012 Oct;18(10):1511-7
Nature medicine 2012 Oct;18(10):1511-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cyclophilin B (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Cyclophilin B Polyclonal Antibody, AlexaFluor 488 conjugate (Product # PA1-027A-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
