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LearnPA1-027A-A555
antibody from Invitrogen Antibodies
Targeting: PPIB
B, CYP-S1, CYPB, OI9, PPIase, SCYLP
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA1-027A-A555 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclophilin B Polyclonal Antibody, Alexa Fluor™ 555
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1027AA555 detects cyclophilin B (CyPB) in mouse, rat, canine, and human tissues.
- Concentration
- 1 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of Cyclophilin B (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Cyclophilin B Polyclonal Antibody, AlexaFluor 555 conjugate (Product # PA1-027A-A555) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
