F54511

antibody from NSJ Bioreagents

Targeting: PPIB B, CYP-S1, CYPB, OI9, PPIase, SCYLP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54511

Provider

NSJ Bioreagents

Product name

CYPB Antibody / Cyclophilin B

Antibody type

Polyclonal

Description

This highly specific CYPB antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.05 ml, 0.2 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the CYPB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human HepG2, 3) human A431, 4) mouse Ba/F3, 5) mouse NIH 3T3 and 6) rat PC-12 cell lysate with CYPB antibody. Expected molecular weight 19-23 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human placental tissue with CYPB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human HeLa cells with CYPB antibody; Blue=isotype control, Green= CYPB antibody.