Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA328627 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-STIM1 (extracellular)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-STIM1 (extracellular)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- STIM1
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of RBL (lanes 1 and 4), HL-60 (lanes 2 and 5), and Jurkat (lanes 3 and 6) cell lysates: 1, 2, 3. Anti-STIM1 (extracellular) antibody, (1:1000). 4, 5, 6. Anti-STIM1 (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of STIM1 in RBL cells. Immunocytochemical staining of STIM1 in live rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells with Anti-STIM1 (extracellular) antibody, (1:50) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of STIM1 in rat stomach. Immunohistochemical staining of paraffin embedded rat stomach sections using Anti-STIM1 (extracellular) antibody, (1:100). STIM1 is expressed in the parietal cells of the gastric mucosa (arrows). Hematoxilin is used as the counterstain.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Indirect flow cytometry analysis of intact RBL (A) and Jurkat (B) cells. black line, Cells + FITC-conjugated goat anti-rabbit antibody. green line, Cells + Anti-STIM1 (extracellular) antibody, (5-10 ug antibody/0.5-1x106 cells) + FITC-conjugated goat anti-rabbit antibody.
- Validation comment
- FC