PA5-17145

antibody from Invitrogen Antibodies

Targeting: STIM1 D11S4896E, GOK

Immunoprecipitation

Antibody data

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Validation data

STIM1 protein structure - PA5-17145 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of STIM1 in extracts from NIH/3T3 and PC12 cells using STIM1 polyclonal antibody (Product # PA5-17145).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of STIM1 was achieved by transfecting PANC-1 with STIM1 specific siRNAs (Silencer® select Product # s531229, s531227). Western blot analysis (Fig. a) was performed using whole cell extracts from the STIM1 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with STIM1 Polyclonal Antibody (Product # PA5-17145, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to STIM1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-STIM1 Polyclonal Antibody (Product # PA5-17145) and a ~83kDa band corresponding STIM1 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of PANC-1 (Lane 1), tissue extracts (30ug lysate) of Mouse Brain (Lane 2), Rat Skeletal Muscle (Lane 3) and Mouse Skeletal Muscle (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).