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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-20371 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STIM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is mouse thymus tissue lysate.
- Concentration
- 1 mg/mL
Submitted references Mitochondrial Transplantation Ameliorates the Development and Progression of Osteoarthritis.
Lee AR, Woo JS, Lee SY, Na HS, Cho KH, Lee YS, Lee JS, Kim SA, Park SH, Kim SJ, Cho ML
Immune network 2022 Apr;22(2):e14
Immune network 2022 Apr;22(2):e14
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse thymus tissue lysate using a STIM1 polyclonal antibody (Product # PA5-20371) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of STIM1 in mouse thymus tissue lysate with STIM1 Polyclonal Antibody (Product # PA5-20371) at (A) 1 and (B) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of STIM1 in Hek293 cells with STIM1 Polyclonal Antibody (Product # PA5-20371) at 20 µg/mL. Red: STIM1 Blue: DAPI staining
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of STIM1 in mouse spleen tissue with STIM1 Polyclonal Antibody (Product # PA5-20371) at 20 µg/mL. Green: STIM1 Blue: DAPI staining
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of STIM1 in human spleen tissue with STIM1 Polyclonal Antibody (Product # PA5-20371) at 2.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of STIM1 in mouse spleen tissue with STIM1 Polyclonal Antibody (Product # PA5-20371) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Attenuation of OA by mitochondrial transplantation. (A) Bar graph shows the amount of isolated mitochondria from 1x10 6 of L6 cells. (B) Representative immunoblots show the expression of Ki-67, STIM1, tubulin, and COX4 in the nuclear, ER, cytosolic, and mitochondrial fractions, respectively. (C) Isolated mitochondira stained with NAO. Representative FACS plot shows percentage of NAO positive mitochondria. (D) Bar graph shows average ATP content of isolated mitochondria. MIA-induced OA rats were observed and evaluated for 29 days (n=5 animals per group). The mitochondrial injection was performed intra-articularly twice weekly. Kinetics of PWL, PWT (E), and weight-bearing (F). (G) Bar graph shows the average amount of CTX-II in serum of each group. (H) Representative images of Safranin O-stained cartilage. Scale bar = 100 mum. (I) Average total Mankin score (left) and OARIS score (right). (J) Representative micro CT images of bone. (K) Percentage of bone surface (left) and BV: TV ratio (right). Data are means +- SEM. NAO, nonyl acridine orange. * p
