LS-B10898

antibody from LSBio

Targeting: HLA-A

Western blot (Enhanced validation)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B10898

Provider

LSBio

Product name

IHC-plus™ HLA-A Antibody LS-B10898

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE HLA-A antibody diluted at 1:1000

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IP samples: A549 whole cell extract A. Control with 4 µg of preimmune Rabbit IgG B. Immunoprecipitation of HLA-A protein by 4 µg HLA-A antibody 10 % SDS-PAGE The immunoprecipitated HLA-A protein was detected by HLA-A antibody diluted at 1:500.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IP samples: A549 whole cell extract A. Control with 4 µg of preimmune Rabbit IgG B. Immunoprecipitation of HLA-A protein by 4 µg HLA-A antibody 10 % SDS-PAGE The immunoprecipitated HLA-A protein was detected by HLA-A antibody diluted at 1:500.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IP samples: A549 whole cell extract A. Control with 4 µg of preimmune Rabbit IgG B. Immunoprecipitation of HLA-A protein by 4 µg HLA-A antibody 10 % SDS-PAGE The immunoprecipitated HLA-A protein was detected by HLA-A antibody diluted at 1:500.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IP samples: A549 whole cell extract A. Control with 4 µg of preimmune Rabbit IgG B. Immunoprecipitation of HLA-A protein by 4 µg HLA-A antibody 10 % SDS-PAGE The immunoprecipitated HLA-A protein was detected by HLA-A antibody diluted at 1:500.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-HLA-A antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-HLA-A antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.