Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 15-9983-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-HLA-ABC Monoclonal Antibody (W6/32), PE-Cyanine5, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey. Applications Reported: The W6/32 antibody has been reported for use in flow cytometric analysis. Applications Tested: This W6/32 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- W6/32
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Hypoxia and hypoxia-inducible factor (HIF) downregulate antigen-presenting MHC class I molecules limiting tumor cell recognition by T cells.
Identification and validation of multiple cell surface markers of clinical-grade adipose-derived mesenchymal stromal cells as novel release criteria for good manufacturing practice-compliant production.
Screening of candidate G-quadruplex ligands for the human c-KIT promotorial region and their effects in multiple in-vitro models.
Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.
ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
Steric shielding of surface epitopes and impaired immune recognition induced by the ebola virus glycoprotein.
Antigen-presenting cells represent targets for R5 HIV-1 infection in the first trimester pregnancy uterine mucosa.
High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss.
A study of human leukocyte antigen G expression in hydatidiform moles.
Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast.
Sethumadhavan S, Silva M, Philbrook P, Nguyen T, Hatfield SM, Ohta A, Sitkovsky MV
PloS one 2017;12(11):e0187314
PloS one 2017;12(11):e0187314
Identification and validation of multiple cell surface markers of clinical-grade adipose-derived mesenchymal stromal cells as novel release criteria for good manufacturing practice-compliant production.
Camilleri ET, Gustafson MP, Dudakovic A, Riester SM, Garces CG, Paradise CR, Takai H, Karperien M, Cool S, Sampen HJ, Larson AN, Qu W, Smith J, Dietz AB, van Wijnen AJ
Stem cell research & therapy 2016 Aug 11;7(1):107
Stem cell research & therapy 2016 Aug 11;7(1):107
Screening of candidate G-quadruplex ligands for the human c-KIT promotorial region and their effects in multiple in-vitro models.
Zorzan E, Da Ros S, Musetti C, Shahidian LZ, Coelho NF, Bonsembiante F, Létard S, Gelain ME, Palumbo M, Dubreuil P, Giantin M, Sissi C, Dacasto M
Oncotarget 2016 Apr 19;7(16):21658-75
Oncotarget 2016 Apr 19;7(16):21658-75
Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.
Fernandez I, Ooi TP, Roy K
Stem cells (Dayton, Ohio) 2014 Jan;32(1):93-104
Stem cells (Dayton, Ohio) 2014 Jan;32(1):93-104
ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
Weissmüller S, Semmler LY, Kalinke U, Christians S, Müller-Berghaus J, Waibler Z
Blood 2012 Jun 28;119(26):6268-77
Blood 2012 Jun 28;119(26):6268-77
Steric shielding of surface epitopes and impaired immune recognition induced by the ebola virus glycoprotein.
Francica JR, Varela-Rohena A, Medvec A, Plesa G, Riley JL, Bates P
PLoS pathogens 2010 Sep 9;6(9):e1001098
PLoS pathogens 2010 Sep 9;6(9):e1001098
Antigen-presenting cells represent targets for R5 HIV-1 infection in the first trimester pregnancy uterine mucosa.
Marlin R, Nugeyre MT, de Truchis C, Berkane N, Gervaise A, Barré-Sinoussi F, Menu E
PloS one 2009 Jun 22;4(6):e5971
PloS one 2009 Jun 22;4(6):e5971
High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss.
Koene GJ, Arts-Hilkes YH, van Dijk AJ, van der Ven KJ, Slootweg PJ, de Weger RA, Tilanus MG
Human immunology 2004 Dec;65(12):1455-62
Human immunology 2004 Dec;65(12):1455-62
A study of human leukocyte antigen G expression in hydatidiform moles.
Goldman-Wohl D, Ariel I, Greenfield C, Hochner-Celnikier D, Lavy Y, Yagel S
American journal of obstetrics and gynecology 2001 Aug;185(2):476-80
American journal of obstetrics and gynecology 2001 Aug;185(2):476-80
Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast.
Stern PL, Beresford N, Friedman CI, Stevens VC, Risk JM, Johnson PM
Journal of immunology (Baltimore, Md. : 1950) 1987 Feb 15;138(4):1088-91
Journal of immunology (Baltimore, Md. : 1950) 1987 Feb 15;138(4):1088-91
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control PE-Cyanine5 (Product # 15-4724-81) (blue histogram) or Anti-Human HLA-ABC PE-Cyanine5 (purple histogram).Cells in the lymphocyte gate were used for analysis.