Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-5754-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-HLA-A3 Monoclonal Antibody (GAP.A3), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, which is a member of the HLA-A family. HLA-A3 exists as two subtypes, HLA-A3.1 and HLA-A3.2, which differ at amino acid positions 152 and 156. MHC class I molecules are expressed on all nucleated cells. These molecules are highly polymorphic, cell surface glycoproteins that non-covalently associate with B2 microglobulin. MHC Class I molecules present intracellular peptides on the cell surface and nonnative peptides are recognized by circulating cytotoxic T lymphocytes. HLA-A3 is expressed by 15-25% of cancer patients and has been demonstrated to bind the tumor-associated antigen human telomerase reverse transcriptase (hTERT). Applications Reported: This GAP.A3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This GAP.A3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GAP.A3
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Donor-Derived Exosomes With Lung Self-Antigens in Human Lung Allograft Rejection.
The specificity of trimming of MHC class I-presented peptides in the endoplasmic reticulum.
Early phagosomes in dendritic cells form a cellular compartment sufficient for cross presentation of exogenous antigens.
Characterization of HLA-A3-restricted cytotoxic T lymphocytes reactive against the widely expressed tumor antigen telomerase.
Analysis of the molecular basis of HLA-A3 recognition by cytotoxic T cells using defined mutants of the HLA-A3 molecule.
Human cytotoxic T-lymphocyte recognition of an HLA-A3 gene product expressed on murine L cells: the only human gene product required on the target cells for lysis is the class I heavy chain.
Gunasekaran M, Xu Z, Nayak DK, Sharma M, Hachem R, Walia R, Bremner RM, Smith MA, Mohanakumar T
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2017 Feb;17(2):474-484
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2017 Feb;17(2):474-484
The specificity of trimming of MHC class I-presented peptides in the endoplasmic reticulum.
Hearn A, York IA, Rock KL
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5526-36
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5526-36
Early phagosomes in dendritic cells form a cellular compartment sufficient for cross presentation of exogenous antigens.
Ackerman AL, Kyritsis C, Tampé R, Cresswell P
Proceedings of the National Academy of Sciences of the United States of America 2003 Oct 28;100(22):12889-94
Proceedings of the National Academy of Sciences of the United States of America 2003 Oct 28;100(22):12889-94
Characterization of HLA-A3-restricted cytotoxic T lymphocytes reactive against the widely expressed tumor antigen telomerase.
Vonderheide RH, Anderson KS, Hahn WC, Butler MO, Schultze JL, Nadler LM
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Nov;7(11):3343-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Nov;7(11):3343-8
Analysis of the molecular basis of HLA-A3 recognition by cytotoxic T cells using defined mutants of the HLA-A3 molecule.
Jelachich ML, Cowan EP, Turner RV, Coligan JE, Biddison WE
Journal of immunology (Baltimore, Md. : 1950) 1988 Aug 15;141(4):1108-13
Journal of immunology (Baltimore, Md. : 1950) 1988 Aug 15;141(4):1108-13
Human cytotoxic T-lymphocyte recognition of an HLA-A3 gene product expressed on murine L cells: the only human gene product required on the target cells for lysis is the class I heavy chain.
Cowan EP, Coligan JE, Biddison WE
Proceedings of the National Academy of Sciences of the United States of America 1985 Jul;82(13):4490-4
Proceedings of the National Academy of Sciences of the United States of America 1985 Jul;82(13):4490-4
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (blue histogram) or Anti-Human HLA-A3 APC (purple histogram). Cells in the lymphocyte gate were used for analysis.