APC-042-200UL
antibody from Invitrogen Antibodies
Targeting: KCNJ12
hIRK1, IRK2, KCNJN1, Kir2.2, Kir2.2v
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- APC-042-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Kir2.2 (KCNJ12) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.39 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotanalysisof rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes: - 1,2. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:200). 3,4. Anti-Kir2.2 (KCNJ12) Antibody , preincubated with Kir2.2/KCNJ12 Blocking Peptide (#BLP-PC042).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression ofKir2.2in ratcerebellum. Immunohistochemicalstaining of perfusion-fixed frozenratbrainsections with Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A.Kir2.2immunoreactivity (green)appeared inBergmann glial fibers (right-pointing arrows) and outlines of molecular layer neurons (left-pointing arrows).B. Pre-incubation of the Antibody with Kir2.2/KCNJ12Blocking Peptide (BLP-PC042), suppressed staining. Cell nuclei are stained with DAPI (blue).MOL = molecular layer.