600-401-919S

antibody from Rockland Immunochemicals, Inc.

Targeting: SMAD3 HsT17436, JV15-2, MADH3

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-919S

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-919S, RRID:AB_11183329

Product name

Anti-SMAD3 pS423 pS425 (RABBIT) Antibody - 600-401-919S

Antibody type

Polyclonal

Vial size

25 µl

References

Submitted references

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Deletion of Akt1 Promotes Kidney Fibrosis in a Murine Model of Unilateral Ureteral Obstruction.

Kim IY, Lee MY, Park MW, Seong EY, Lee DW, Lee SB, Bae SS, Kim SS, Song SH
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TGF-β-induced IGFBP-3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion.

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Kang H, Jha S, Ivovic A, Fratzl-Zelman N, Deng Z, Mitra A, Cabral WA, Hanson EP, Lange E, Cowen EW, Katz J, Roschger P, Klaushofer K, Dale RK, Siegel RM, Bhattacharyya T, Marini JC
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Inhibition of Non-Small Cell Lung Cancer Cells by Oxy210, an Oxysterol-Derivative that Antagonizes TGFβ and Hedgehog Signaling.

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βklotho is essential for the anti-endothelial mesenchymal transition effects of N-acetyl-seryl-aspartyl-lysyl-proline.

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American journal of physiology. Renal physiology 2015 Aug 1;309(3):F204-15

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SnoN suppresses maturation of chondrocytes by mediating signal cross-talk between transforming growth factor-β and bone morphogenetic protein pathways.

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Coffman JA, Coluccio A, Planchart A, Robertson AJ
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TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta.

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Molecular cell 2008 Sep 26;31(6):918-24

Immunohistochemistry

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Rockland's affinity purified anti-Smad3 pS423 pS425 antibody was used at 2.5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Validation comment

Immunohistochemistry