Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-77483 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHRM4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.85 mg/mL
- Storage
- -20°C
Submitted references Nerve growth factor interacts with CHRM4 and promotes neuroendocrine differentiation of prostate cancer and castration resistance.
Chen WY, Wen YC, Lin SR, Yeh HL, Jiang KC, Chen WH, Lin YS, Zhang Q, Liew PL, Hsiao M, Huang J, Liu YN
Communications biology 2021 Jan 4;4(1):22
Communications biology 2021 Jan 4;4(1):22
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membrane (lanes 1 and 2), mouse brain membrane (lane 3 and 5) and SH-SY5Y cell lystae (lanes 4 and 6) with CHRM4 polyclonal antibody (Product # PA5-77483) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CHRM4 in paraffin-embedded C6 cells. A) Samples were probed with CHRM4 polyclonal antibody (Product # PA5-77483) using a dilution of 1:100, and incubated with goat-anti-rabbit-AlexaFluor-594 and DAPI. B) Non-transfected cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHRM4 in mouse islands of Calleja. A) Samples were probed with CHRM4 polyclonal antibody (Product # PA5-77483) and incubated with DAPI. B) Nuclei stained image. C) Merged image of Panels A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 Positive associations among the NGF, CHRM4, and neuroendocrine markers in clinical samples. a, b IHC staining ( a ) and relative intensities ( b ) of the NGF and CHRM4 of a prostate cancer TMA consisting of normal tissues ( n = 16), adenocarcinomas with a Gleason score of =8 ( n = 19), and SCNCs ( n = 14) from Duke University School of Medicine. Scale bars, 100 mum. Data are presented as the mean +- SEM. * vs. normal tissues. * p < 0.05, **** p < 0.0001; by a two-way ANOVA. c Immunofluorescence staining of the tissue microarray (TMA) with antibodies for the CHRM4 (red) and NGF (green). Nuclei were visualized with DAPI staining (blue). Scale bars represent 20 um. d Mean mRNA expression levels of the NGF and CHRM4 in human normal prostate ( n = 28), primary ( n = 98), and metastatic ( n = 13) prostate cancer samples from the Taylor prostate cancer dataset . * vs. normal tissues. * p < 0.05, ** p < 0.01; by a two-way ANOVA. e Mean mRNA expressions of the NGF and CHRM4 in patient samples in the Taylor prostate cancer dataset by Gleason scores. * vs. Gleason score of 6. Significance was determined by a one-way ANOVA. f , g Correlation analysis of NGF and CHRM4 mRNA levels with PSA levels ( f ) and correlation analysis of NGF and ZBTB46 mRNA levels with CHRM4 mRNA levels ( g ) in prostate tissue samples from the Taylor prostate cancer dataset . R correlation coefficient, P p (two-tailed) value. Data were tested by corre