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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-32000 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MANEA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa, HepG2. Predicted reactivity: Mouse (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MANEA Polyclonal Antibody (Product # PA5-32000). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: HeLa. Lane C: HepG2. 10% SDS PAGE. MANEA Polyclonal Antibody (Product # PA5-32000) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of MANEA was performed in paraffin-embedded mouse liver tissue using MANEA Polyclonal Antibody (Product # PA5-32000) at a dilution of 1:500.
