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LearnPA5-111857
antibody from Invitrogen Antibodies
Targeting: NECTIN3
CD113, CDw113, DKFZP566B0846, nectin-3, PPR3, PVRL3, PVRR3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-111857 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nectin 3 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Nectin 3 was performed in human U-87 MG glioblastoma cell line lysate. Lane 1: Nectin 3 (extracellular) Antibody (Product # PA5-111857) at a dilution of 1:200. Lane 2: Nectin 3 (extracellular) Antibody preincubated with the negative control antigen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Nectin 3 was performed in mouse (lanes 1 and 3) and rat (lanes 2 and 4) testis membranes. Lane 1,2: Nectin 3 (extracellular) Antibody (Product # PA5-111857) at a dilution of 1:200. Lane 3,4: Nectin 3 (extracellular) Antibody preincubated with the negative control antigen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Nectin 3 was performed in rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate. Lane 1,2: Nectin 3 (extracellular) Antibody (Product # PA5-111857) at a dilution of 1:200. Lane 3,4: Nectin 3 (extracellular) Antibody preincubated with the negative control antigen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nectin 3 in perfusion-fixed, frozen rat parietal cortex tissue sections using Nectin 3 (extracellular) Antibody (Product # PA5-111857) at a dilution of 1:100, followed by goat anti-rabbit-AlexaFluor-488. Nectin-3 immunoreactivity (green) appears in cells of the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Nectin 3 in live intact human Jurkat T-cell leukemia cells. Black: Cells. Pink: Cells and goat-anti-rabbit-FITC. Green: Cells, Nectin 3 (extracellular) Antibody (Product # PA5-111857), and goat-anti-rabbit-FITC.
