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LearnANR-053-200UL
antibody from Invitrogen Antibodies
Targeting: NECTIN3
CD113, CDw113, DKFZP566B0846, nectin-3, PPR3, PVRL3, PVRR3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ANR-053-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nectin-3/PVRL3 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)testis membranes: - 1,2. Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053), (1:200).3,4. Anti-Nectin-3/PVRL3 (extracellular) Antibody , preincubated with Nectin-3/PVRL3 (extracellular) Blocking Peptide (#BLP-NR053).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human U-87 MG glioblastoma cell line lysate: - 1. Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053), (1:200). 2. Anti-Nectin-3/PVRL3 (extracellular) Antibody , preincubated with Nectin-3/PVRL3 (extracellular) Blocking Peptide (#BLP-NR053).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)brain lysate: - 1,2. Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053), (1:200).3,4. Anti-Nectin-3/PVRL3 (extracellular) Antibody , preincubated with Nectin-3/PVRL3 (extracellular) Blocking Peptide (#BLP-NR053).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Nectin-3 in rat parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053), (1:100), followed by goat Anti-rabbit-AlexaFluor-488. Nectin-3 immunoreactivity (green) appears in cells of the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of Nectin-3 in live intact human Jurkat T-cell leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053), (2.5 µg) + goat- Anti-rabbit-FITC.
