F55131-0.4ML

antibody from NSJ Bioreagents

Targeting: RSPO2 MGC35555

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F55131-0.4ML

Provider

NSJ Bioreagents

Product name

RSPO2 Antibody / R-spondin-2

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human

Host

Rabbit

Vial size

0.4 ml, 0.08 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Store at 4oC for up to one month. For long term, aliquot the RSPO2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HEK293 cell lysate with RSPO2 antibody. Predicted molecular weight ~28 kDa, ~20 kDa and ~21 kDa (three isoforms).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung carcinoma tissue with RSPO2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HEK293 cells with RSPO2 antibody; Blue=isotype control, Green= RSPO2 antibody.