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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-93314 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-93314, RRID:AB_1237295
- Product name
- Rabbit Polyclonal PHF8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The HIF/PHF8/AR axis promotes prostate cancer progression.
PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis.
Tong D, Liu Q, Liu G, Yuan W, Wang L, Guo Y, Lan W, Zhang D, Dong S, Wang Y, Xiao H, Mu J, Mao C, Wong J, Jiang J
Oncogenesis 2016 Dec 19;5(12):e283
Oncogenesis 2016 Dec 19;5(12):e283
PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis.
Zhu Z, Wang Y, Li X, Wang Y, Xu L, Wang X, Sun T, Dong X, Chen L, Mao H, Yu Y, Li J, Chen PA, Chen CD
Cell research 2010 Jul;20(7):794-801
Cell research 2010 Jul;20(7):794-801
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHF8 Antibody [NB100-93314] - Detection of Human PHF8 on HeLa whole cell lysate using NB100-93314. PHF8 was also immunoprecipitated by rabbit anti-PHF8 antibody NB100-93313.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHF8 Antibody [NB100-93314] - Detection of Human PHF8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, HeLa (RIPA), 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PHF8 antibody NB100-93314 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93314] - Section of human prostate carcinoma (left) and mouse teratoma (right). Dilution: 1:200 (1 ug/ml).
