13174-1-AP

antibody from Proteintech Group

Targeting: DNAJB1 Hsp40, HSPF1, RSPH16B, Sis1

Provider product page for 13174-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [28]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 13174-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#13174-1-AP, RRID:AB_2230742
Product name: DNAJB1 antibody
Antibody type: Polyclonal
Description: KD/KO validated DNAJB1 antibody (Cat. #13174-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

DNAJB1 protein structure - 13174-1-AP shown in red.

Submitted references An ATP13A1-assisted topogenesis pathway for folding multi-spanning membrane proteins.

Ji J, Cui MK, Zou R, Wu MZ, Ge MX, Li J, Zhang ZR
Molecular cell 2024 May 16;84(10):1917-1931.e15

Reactive metabolite of trovafloxacin activates inflammasomes: Implications for trovafloxacin-induced liver injury.

Tanaka S, Noda T, Urashima K, Ijiri Y, Kohda Y, Kato R
Journal of applied toxicology : JAT 2024 Jun;44(6):846-852

Targeting HSP70 chaperones by rhein sensitizes liver cancer to artemisinin derivatives.

Wang J, Zhang J, Guo Z, Hua H, Zhang H, Liu Y, Jiang Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Jan;122:155156

Disrupted HSF1 regulation in normal and exceptional brain aging.

Trivedi R, Knopf B, Rakoczy S, Manocha GD, Brown-Borg H, Jurivich DA
Biogerontology 2024 Feb;25(1):147-160

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination.

Li L, Li D, Sun D, Zhang X, Lei W, Wu M, Huang Q, Nian X, Dai W, Lu X, Zhou Z, Zhu Y, Xiao Y, Zhang L, Mo W, Liu Z, Zhang L
Developmental cell 2023 Nov 6;58(21):2275-2291.e6

A curcumin analogue GO-Y030 depletes cancer stem cells by inhibiting the interaction between the HSP70/HSP40 complex and its substrates.

Suzuki M, Yamamoto Y, Nishijima-Matsunobu A, Kawasaki Y, Shibata H, Omori Y
FEBS open bio 2023 Mar;13(3):434-446

Heat Shock Protein Family A Member 1 Promotes Intracellular Amplification of Hepatitis B Virus Covalently Closed Circular DNA.

Tang L, An P, Zhao Q, Winkler CA, Chang J, Guo JT
Journal of virology 2023 Jan 31;97(1):e0126122

Mechanism of non-steroidal anti-androgen-induced liver injury: Reactive metabolites of flutamide and bicalutamide activate inflammasomes.

Kato R, Yamada T, Noda T, Tanaka S, Kohda Y, Ijiri Y
Toxicology in vitro : an international journal published in association with BIBRA 2023 Aug;90:105606

Integrative proteomics and m6A microarray analyses of the signatures induced by METTL3 reveals prognostically significant in gastric cancer by affecting cellular metabolism.

Peng G, Chen S, Zheng N, Tang Y, Su X, Wang J, Dong R, Wu D, Hu M, Zhao Y, Liu M, Wu H
Frontiers in oncology 2022;12:996329

Defective Human SRP Induces Protein Quality Control and Triggers Stress Response.

Tikhonova EB, Gutierrez Guarnizo SA, Kellogg MK, Karamyshev A, Dozmorov IM, Karamysheva ZN, Karamyshev AL
Journal of molecular biology 2022 Nov 30;434(22):167832

Regorafenib and reactive metabolite of sunitinib activate inflammasomes: Implications for multi tyrosine kinase inhibitor-Iiduced immune related adverse events.

Kato R, Ijiri Y
Die Pharmazie 2022 Feb 1;77(2):54-58

The Therapeutic Roles of Recombinant Hsp90α on Cornea Epithelial Injury.

Wang M, Hu J, Qu J, Huang H, Zhang J, Zhang J, Li H, Cui X, Zhang F, Hu MY, Li J, Hu Y
Investigative ophthalmology & visual science 2022 Feb 1;63(2):30

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin.

Alalem M, Bhosale M, Ranjan A, Yamamoto S, Kaida A, Nishikawa S, Parrales A, Farooki S, Anant S, Padhye S, Iwakuma T
Cancers 2022 Aug 29;14(17)

Identification of a HTT-specific binding motif in DNAJB1 essential for suppression and disaggregation of HTT.

Ayala Mariscal SM, Pigazzini ML, Richter Y, Özel M, Grothaus IL, Protze J, Ziege K, Kulke M, ElBediwi M, Vermaas JV, Colombi Ciacchi L, Köppen S, Liu F, Kirstein J
Nature communications 2022 Aug 10;13(1):4692

Role of caspase-8 and/or -9 as biomarkers that can distinguish the potential to cause toxic- and immune related-adverse event, for the progress of acetaminophen-induced liver injury.

Noda T, Kato R, Hattori T, Furukawa Y, Ijiri Y, Tanaka K
Life sciences 2022 Apr 1;294:120351

PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway.

Li Y, Kang J, Fu J, Luo H, Liu Y, Li Y, Sun L
International journal of molecular sciences 2021 Mar 3;22(5)

Local translation in nuclear condensate amyloid bodies.

Theodoridis PR, Bokros M, Marijan D, Balukoff NC, Wang D, Kirk CC, Budine TD, Goldsmith HD, Wang M, Audas TE, Lee S
Proceedings of the National Academy of Sciences of the United States of America 2021 Feb 16;118(7)

Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction.

Pan J, Ma N, Zhong J, Yu B, Wan J, Zhang W
Molecular therapy. Nucleic acids 2021 Dec 3;26:970-986

Augmentation of the heat shock axis during exceptional longevity in Ames dwarf mice.

Trivedi R, Knopf B, Tripathi JK, Rakoczy S, Manocha GD, Brown-Borg H, Jurivich DA
GeroScience 2021 Aug;43(4):1921-1934

Amiodarone, Unlike Dronedarone, Activates Inflammasomes via Its Reactive Metabolites: Implications for Amiodarone Adverse Reactions.

Kato R, Ijiri Y, Hayashi T
Chemical research in toxicology 2021 Aug 16;34(8):1860-1865

Reactive metabolite of gefitinib activates inflammasomes: implications for gefitinib-induced idiosyncratic reaction.

Kato R, Ijiri Y, Hayashi T, Uetrecht J
The Journal of toxicological sciences 2020;45(11):673-680

Deletion of the Capn1 Gene Results in Alterations in Signaling Pathways Related to Alzheimer's Disease, Protein Quality Control and Synaptic Plasticity in Mouse Brain.

Su W, Zhou Q, Wang Y, Chishti A, Li QQ, Dayal S, Shiehzadegan S, Cheng A, Moore C, Bi X, Baudry M
Frontiers in genetics 2020;11:334

Multifactorial Attenuation of the Murine Heat Shock Response With Age.

Jurivich DA, Manocha GD, Trivedi R, Lizakowski M, Rakoczy S, Brown-Borg H
The journals of gerontology. Series A, Biological sciences and medical sciences 2020 Sep 25;75(10):1846-1852

Crosstalk Between Chaperone-Mediated Protein Disaggregation and Proteolytic Pathways in Aging and Disease.

Feleciano DR, Juenemann K, Iburg M, Brás IC, Holmberg CI, Kirstein J
Frontiers in aging neuroscience 2019;11:9

The 2-Hydroxyiminostilbene Metabolite of Carbamazepine or the Supernatant from Incubation of Hepatocytes with Carbamazepine Activates Inflammasomes: Implications for Carbamazepine-Induced Hypersensitivity Reactions.

Kato R, Ijiri Y, Hayashi T, Uetrecht J
Drug metabolism and disposition: the biological fate of chemicals 2019 Oct;47(10):1093-1096

Inhibition of Heat Shock Protein 90 by 17-AAG Reduces Inflammation via P2X7 Receptor/NLRP3 Inflammasome Pathway and Increases Neurogenesis After Subarachnoid Hemorrhage in Mice.

Zuo Y, Wang J, Liao F, Yan X, Li J, Huang L, Liu F
Frontiers in molecular neuroscience 2018;11:401

HSP40 interacts with pyruvate kinase M2 and regulates glycolysis and cell proliferation in tumor cells.

Huang L, Yu Z, Zhang T, Zhao X, Huang G
PloS one 2014;9(3):e92949

Identification of Hsf1 as a novel androgen receptor-regulated gene in mouse Sertoli cells.

Yang L, Wang Y, Zhang Q, Lai Y, Li C, Zhang Q, Huang W, Duan Y, Jiang Z, Li X, Cai Z, Mou L, Gui Y
Molecular reproduction and development 2014 Jun;81(6):514-23

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13174-1-AP(DNAJB1 antibody) at dilution of 1:600
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using DNAJB1 antibody 13174-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line