PA5-17382

antibody from Invitrogen Antibodies

Targeting: DNAJB1 Hsp40, HSPF1, RSPH16B, Sis1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17382

Provider

Invitrogen Antibodies

Product name

HSP40 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with other HSPs.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

35 µg/mL

Storage

-20°C

References

Submitted references

The interplay between redox signalling and proteostasis in neurodegeneration: In vivo effects of a mitochondria-targeted antioxidant in Huntington's disease mice.

Pinho BR, Duarte AI, Canas PM, Moreira PI, Murphy MP, Oliveira JMA
Free radical biology & medicine 2020 Jan;146:372-382

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HSP40 in HeLa cells, heat-treated, using a HSP40 polyclonal antibody (Product # PA5-17382) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HSP40 was performed using 70% confluent log phase HeLa cells heat shocked at 43°C for 1 hr. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSP40 Polyclonal Antibody (Product # PA5-17382) at 1:400 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear translocation upon heat shock. Panel e shows untreated HeLa cells with cytoplasmic localization. Panel f represents control cells with no primary to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 Levels of mitochondrial biomarkers and chaperones in WT and R6/2 mice. Western blot quantifications in brain, liver and muscle samples from WT and R6/2 mice treated with vehicle or MitoQ. (A) Mitochondrial biomarkers: i ) SDHA, succinate dehydrogenase complex, subunit A; ii ) TFAM, mitochondrial transcription factor A, iii ) Hsp60; and iv ) representative blots. (B) Cytosolic chaperones: i ) Hsp70; ii ) Hsp40; iii ) Hsp90; and iv ) representative blots. Coom - Coomassie staining loading control. Data are in percentage of the mean of WT-vehicle (control); n = 5; * P < 0.05, genotype effect. Fig. 3